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DLAT Rabbit Polyclonal Antibody#53872

DLAT Rabbit Polyclonal Antibody
DLAT Rabbit Polyclonal Antibody
DLAT Rabbit Polyclonal Antibody
DLAT Rabbit Polyclonal Antibody
DLAT Rabbit Polyclonal Antibody
是否有货: Yes
产品总价:
产品详情

产品名称DLAT Rabbit Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化Affinity purification

应用WB,IHC,IF

种属反应性Human,Mouse,Rat

免疫原描述Recombinant fusion protein of human DLAT (NP_001922.2).

别名DLAT;DLTA;PDC-E2;PDCE2

数据库入口号Swiss Prot:P10515
GeneID:1737

UniprotP10515

计算分子量68kDa

实际分子量69kDa

配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

保存Store at -20˚C. Avoid freeze / thaw cycles.

应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded rat kidney using DLAT at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using DLAT at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using DLAT at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DLAT at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using DLAT at dilution of 1:100. Blue: DAPI for nuclear staining.
背景
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com