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位置: 首页 > 磷酸化抗体 > VASP(Phospho-Ser157) Antibody

VASP(Phospho-Ser157) Antibody#11214

VASP(Phospho-Ser157) Antibody
VASP(Phospho-Ser157) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称VASP(Phospho-Ser157) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Rt

特异性The antibody detects endogenous level of VASP only when phosphorylated at serine 157.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 157 (R-V-S(p)-N-A) derived from Human VASP.

基因/蛋白名称VASP

修饰Phospho

数据库入口号Swiss-Prot: P50552
NCBI Protein: NP_003361.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 50kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from C6 cells untreated or treated with PMA using VASP(Phospho-Ser157) Antibody #11214.
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP(Phospho-Ser157) Antibody #11214(left) or the same antibody preincubated with blocking peptide(right).
背景
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

Zhao WM, et al. (2001) EMBO J 20(9): 2315-2325.
Millard TH, et al. (2005) EMBO J 24(2): 240-250.
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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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