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位置: 首页 > 抗体 > TAF5 Rabbit Polyclonal Antibody

TAF5 Rabbit Polyclonal Antibody#55149

TAF5 Rabbit Polyclonal Antibody
TAF5 Rabbit Polyclonal Antibody
是否有货: Yes
产品总价:
产品详情

产品名称TAF5 Rabbit Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化Affinity purification

应用WB,IF

种属反应性Human,Mouse,Rat

免疫原描述Recombinant fusion protein of human TAF5 (NP_008882.2).

别名TAF5;TAF(II)100;TAF2D;TAFII-100;TAFII100

数据库入口号Swiss Prot:Q15542
GeneID:6877

UniprotQ15542

计算分子量80kDa/86kDa

实际分子量100kDa

配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

保存Store at -20˚C. Avoid freeze / thaw cycles.

应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:100
Western blot analysis of extracts of mouse brain, using TAF5 at 1:1000 dilution.
Immunofluorescence analysis of A-549 cells using TAF5 . Blue: DAPI for nuclear staining.
背景
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com