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位置: 首页 > 抗体 > Cyclin A2 Rabbit Polyclonal Antibody

Cyclin A2 Rabbit Polyclonal Antibody#55266

Cyclin A2 Rabbit Polyclonal Antibody
Cyclin A2 Rabbit Polyclonal Antibody
Cyclin A2 Rabbit Polyclonal Antibody
Cyclin A2 Rabbit Polyclonal Antibody
是否有货: Yes
产品总价:
产品详情

产品名称Cyclin A2 Rabbit Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化Affinity purification

应用WB,IHC,IF

种属反应性Human,Mouse,Rat

免疫原描述A synthetic peptide of human Cyclin A2

别名CCN1;CCNA;Cyclin A2;CCNA2;cyclin-A2

数据库入口号Swiss Prot:P20248
GeneID:890

UniprotP20248

计算分子量48kDa

实际分子量55kDa

配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

保存Store at -20˚C. Avoid freeze / thaw cycles.

应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using Cyclin A2 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Cyclin A2 at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Cyclin A2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Cyclin A2 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
背景
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

如果您使用该产品55266发表了文章,请通知我们,让我们可以引用您的文献。

et al,CD155 Cooperates with PD-1/PD-L1 to Promote Proliferation of Esophageal Squamous Cancer Cells via PI3K/Akt and MAPK Signaling Pathways. In Cancers (Basel) on 2022 Nov 15 by Xiyang Tan, Jie Yang, et al..PMID:36428703, , (2022),
PMID: 36428703
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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