产品详情
产品名称mTOR(Phospho-Ser2448) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of mTOR only when phosphorylated at serine 2448.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 2448 (T-D-S(p)-Y-S) derived from Human mTOR.
基因/蛋白名称mTOR
修饰Phospho
别名FRAP; FRAP1; FRAP2; RAFT1;
数据库入口号Swiss-Prot: P42345
NCBI Protein: NP_004949.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 289kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR(Phospho-Ser2448) Antibody #11221(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using mTOR(Phospho-Ser2448) Antibody #11221.
Western blot analysis of extracts from 293 cells untreatedor treated with PMA using mTOR(Phospho-Ser2448) Antibody #11221.
Immunohistochemical analysis ofparaffin-embedded human breast carcinomatissue using mTOR (Phospho-Ser2448) Antibody #11221
Western blot analysis of extracts from Hela cells, treatedwith IFNa or calf intestinal phosphatase (CIP), using mTOR(Phospho-Ser2448) Antibody #11221.
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