产品详情
产品名称WNT5B Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC
种属反应性Human,Mouse,Rat
免疫原描述Recombinant fusion protein of human WNT5B (NP_116031.1).
别名WNT5B
数据库入口号Swiss Prot:Q9H1J7
GeneID:81029
UniprotQ9H1J7
计算分子量40kDa
实际分子量50kDa
配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using WNT5B at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat bone marrow using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis leydig using WNT5B at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using WNT5B at dilution of 1:100 (40x lens).
背景
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.