产品详情
产品名称DDX50 Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human,Mouse,Rat
免疫原描述Recombinant fusion protein of human DDX50 (NP_076950.1).
别名DDX50;GU2;GUB;RH-II/GuB;mcdrh
数据库入口号Swiss Prot:Q9BQ39
GeneID:79009
UniprotQ9BQ39
计算分子量82kDa
实际分子量105kDa
配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Immunohistochemistry of paraffin-embedded mouse testis using DDX50 at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX50 at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DDX50 at 1:3000 dilution.
Immunofluorescence analysis of C6 cells using DDX50 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDX50 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DDX50 at dilution of 1:100. Blue: DAPI for nuclear staining.
背景
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.