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位置: 首页 > 重组兔单抗 > SPT5 Rabbit mAb

SPT5 Rabbit mAb#52865

评价
Recombinant
SPT5 Rabbit mAb
SPT5 Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称SPT5 Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal antibody

克隆号S01-5C2

亚型IgG

纯化Affinity Purified

应用WB IHC

种属反应性Human,Mouse,Rat

免疫原描述A synthetic peptide of human SPT5

标记Unconjugated

修饰Unmodification

别名SPT5; SPT5H; Tat-CT1

数据库入口号Swiss-Prot:O00267
GeneID:6829

UniprotO00267

Gene ID6829

计算分子量Calculated MW:121 kDa,Observed MW:150 kDa

配方50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

保存Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

应用详情
WB: 1/1000 IHC: 1/50
Western blot detection of SPT5 in Hela,A549,HL-60 cell lysates using SPT5 Rabbit mAb(1:1000 diluted).Predicted band size:121kDa.Observed band size:150kDa.
Immunohistochemistry of SPT5 in paraffin-embedded Human breast cancer tissue using SPT5 Rabbit mAb at dilution 1/50
背景
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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