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位置: 首页 > 重组兔单抗 > NEDD8 Activating Enzyme E1C Rabbit mAb

NEDD8 Activating Enzyme E1C Rabbit mAb#52880

评价
Recombinant
NEDD8 Activating Enzyme E1C Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称NEDD8 Activating Enzyme E1C Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal antibody

克隆号S05-6C8

亚型IgG

纯化Affinity Purified

应用WB

种属反应性Human,Mouse,Rat

免疫原描述A synthetic peptide of human UBA3

标记Unconjugated

修饰Unmodification

别名NAE2; UBE1C; hUBA3

数据库入口号Swiss-Prot:Q8TBC4
GeneID:9039

UniprotQ8TBC4

Gene ID9039

计算分子量Calculated MW:52 kDa,Observed MW:52 kDa

配方50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

保存Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

应用详情
WB: 1/2000-1/10000
Western blot detection of UBA3 in Hela,A549,HL-60 cell lysates using UBA3 Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:52kDa.
背景
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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