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位置: 首页 > 磷酸化抗体 > c-Kit(Phospho-Tyr721) Antibody

c-Kit(Phospho-Tyr721) Antibody#11240

c-Kit(Phospho-Tyr721) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称c-Kit(Phospho-Tyr721) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 721 (N-E-Y(p)-M-D) derived from Human c-Kit.

基因/蛋白名称c-Kit

修饰Phospho

别名KIT , CD117, SCFR, PBT

数据库入口号Swiss-Prot: P10721
NCBI Protein: NP_000213.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells untreated or treated with IFN-a using c-Kit(Phospho-Tyr721) Antibody #11240.
背景
This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Nocka, K. et al. (1990) EMBO J. 9, 1805-1813.
Hirota, S. et al. (1998) Science 279, 577-580.
Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162.
Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253.
Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.

如果您使用该产品11240发表了文章,请通知我们,让我们可以引用您的文献。

et al,S-allyl cysteine ameliorates the quality of sperm and provides protection from age-related sperm dysfunction and oxidative stress in rats.In J Clin Biochem Nutr on 2014 Nov by Shigekazu Takemura, Hiroshi Ichikawa et al..PMID: 25411519, , (2014),
PMID: 25411519
Xiao-ning Gao, Ji Lin, Li Gao et al,MicroRNA-193b regulates c-Kit proto-oncogene and represses cell proliferation in acute myeloid leukemia., Leukemia Research, 35(9):1226-1232 (2011),
PMID: 21724256
et al,MicroRNA-193b regulates c-Kit proto-oncogene and represses cell proliferation in acute myeloid leukemia. In Leuk Res on 2011 Sep by Xiao-ning Gao, Ji Lin, et al..PMID: 21724256 , , (2011),
PMID: 21724256
et al,MicroRNA-193a represses c-kit expression and functions as a methylation-silenced tumor suppressor in acute myeloid leukemia. In Oncogene on 2011 Aug 4 by X-N Gao, J Lin, et al..PMID: 21399664 , , (2011),
PMID: 21399664
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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