产品详情
产品名称CDC2(Phospho-Tyr15) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y(p)-G-V) derived from Human CDC2.
基因/蛋白名称CDC2
修饰Phospho
别名CDC28; CDC2A; CDK1; Cyclin-dependent kinase 1;
数据库入口号Swiss-Prot: P06493
NCBI Protein: NP_001163877.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 34kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Tyr15) Antibody #11244.
Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #11244.
Western blot analysis of extracts from 293 cells, treated with Hydroxyurea or calf intestinal phosphatase (CIP), using CDC2 (Phospho-Tyr15) Antibody #11244.
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