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GAPDH Antibody#24407

GAPDH Antibody
GAPDH Antibody
是否有货: 有货
产品总价:
产品详情

产品名称GAPDH Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Affinity chromatography purified via peptide column

应用ELISA WB IHC

种属反应性Hu Ms Rt

免疫原类型Peptide

免疫原描述Raised against a 16 amino acid peptide from near the amino-terminus of human GAPDH.

基因/蛋白名称GAPDH

别名Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD

数据库入口号P04406

浓度1mg/ml

配方Supplied in PBS containing 0.02% sodium azide.

保存Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at (A) 0.125, (B) 0.25 and (C) 0.5 ug/mL.
Immunohistochemistry of GAPDH in human liver tissue with GAPDH antibody at 10 ug/mL.
背景
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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