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位置: 首页 > 磷酸化抗体 > HSP27(Phospho-Ser82) Antibody

HSP27(Phospho-Ser82) Antibody#11248

HSP27(Phospho-Ser82) Antibody
HSP27(Phospho-Ser82) Antibody
HSP27(Phospho-Ser82) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称HSP27(Phospho-Ser82) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of HSP27 only when phosphorylated at serine 82.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 82 (Q-L-S(p)-S-G) derived from Human HSP27.

基因/蛋白名称HSP27

修饰Phospho

别名CMT2F, HMN2B, HSP27, HSP28, Hsp25

数据库入口号Swiss-Prot: P04792
NCBI Protein: NP_001531.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27(Phospho-Ser82) Antibody #11248.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Phospho-Ser82) Antibody #11248(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from Hela cells, treated with UV or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser82) Antibody #11248.
背景

Involved in stress resistance and actin organization.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com