产品名称Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2, His
来源种属Escherichia coli.
纯化> 97 % by SDS-PAGE and HPLC analyses.
生物活性Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107IU/mg in the presence of actinomycin D.
内毒素Less than 1 EU/ug of rHuTNF-α/TNFSF2, His as determined by LAL method.
别名Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin
UniprotP01375
Gene ID7124
计算分子量Approximately 18.3 kDa, a single, non-glycosylated polypeptide chain containing 164 amino acids with Met and 6 × His at N-terminus.
序列MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
配方Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
3-5days
