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位置: 首页 > 重组蛋白 > Recombinant Human Insulin-like Growth Factor-Binding Protein 4, Insect Cells Derived

Recombinant Human Insulin-like Growth Factor-Binding Protein 4, Insect Cells Derived#AP60065

Recombinant Human Insulin-like Growth Factor-Binding Protein 4, Insect Cells Derived
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Insulin-like Growth Factor-Binding Protein 4, Insect Cells Derived

来源种属Insect Cell

纯化> 97 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.1 μg/ml, corresponding to a specific activity of > 1.0 x104 IU/mg in the presence of 14 ng/ml of rHuIGF-II.

内毒素Less than 0.1 EU/ug of rHuIGF-BP4, Insect Cell as determined by LAL method.

计算分子量The apparent molecular weight is about 30 kDa in SDS-PAGE under reducing conditions, containing 237 amino acids.

序列DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFRE

配方Lyophilized from a 0.2 μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins that maintain IGF-I and IGF-II in the circulation and direct them to their target tissues. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP-4 consistently inhibits several cancer cells in vivo and in vitro.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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