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位置: 首页 > 重组蛋白 > Recombinant Human Interferon-omega

Recombinant Human Interferon-omega#AP60099

Recombinant Human Interferon-omega
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Interferon-omega

来源种属Escherichia coli

纯化> 97 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x108 IU/mg.

内毒素Less than 1 EU/ug of rHuIFN-蠅 as determined by LAL method.

别名Interferon alpha-II-1

UniprotP05000

Gene ID3467

计算分子量Approximately 20.0 kDa, containing 172 amino acid residues with two conserved disulfide bonds.

序列CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS

配方Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Interferon-Omega (IFN-蠅) coded by IFNW1 gene in human, is a number of the type I interferon family, which includes IFN-α, IFN-尾, and IFN-蠅. The IFNAR-1/IFNAR-2 receptor complex can help with the signal transduction, followed the antiviral or the antiproliferative actions. IFN-蠅 is derived from IFN-α/尾 and share 75 % sequence with IFN-α. It has two intramolecular disulfide bonds which are crucial for activities. Mire-Sluis et al have described bioassays for IFN-α, IFN-尾, and IFN-蠅 that exploit the ability of these factors to inhibit proliferation of TF-1 cells induced by GM-CSF. The bioassays can be used also with Epo and TF-1 cells, or Epo and Epo-transfected UT-7 cells.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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