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位置: 首页 > 重组蛋白 > Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3

Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3#AP60102

Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Novel Neurtrophin-1/B-Cell Stimulating Factor-3

来源种属Escherichia coli.

纯化> 98 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of > 2.5 x105 IU/mg in the presence of human CNTF R alpha.

内毒素Less than 0.1 EU/ug of rHuNNT-1/BCSF-3 as determined by LAL method.

别名Cardiotrophin-like cytokine factor 1, BSF-3

UniprotQ9UBD9

Gene ID23529

计算分子量Approximately 22.3 kDa, a single non-glycosylated polypeptide chain containing 198 amino acids.

序列LNRTGDPGPG PSIQKTYDLT RYLEHQLRSL AGTYLNYLGP PFNEPDFNPP RLGAETLPRA TVDLEVWRSL NDKLRLTQNY EAYSHLLCYL RGLNRQAATA ELRRSLAHFC TSLQGLLGSI AGVMAALGYP LPQPLPGTEP TWTPGPAHSD FLQKMDDFWL LKELQTWLWR SAKDFNRLKK KMQPPAAAVT LHLGAHGF

配方Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Novel Neurotrophin-1 (NNT-1) or B-Cell Stimulating Factor-3 (BSF-3), is a new member of the IL-6 family of structurally related cytokines that includes IL-6, CNTF, LIF, CT-1, IL-11 and OSM. All family members share the receptor subunit gp130 that belong to the type I cytokine receptor superfamily. Ligand binding leads to gp130 homodimerization or heterodimerization (with LIF receptor or OSM receptor beta), and induces cell signaling and functional activity. For several family members, including CNTF, IL-6, and IL-11, binding of the ligand to a specific receptor alpha subunit (CNTF R alpha, IL-6 R alpha, or IL-11 R alpha) is required prior to gp130 homo- or hetero-dimerization.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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