产品名称Recombinant Murine Leukemia Inhibitory Factor

来源种属Escherichia coli.

纯化> 98 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 x10⁸ IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.

内毒素Less than 1 EU/ug of rMuLIF as determined by LAL method.

别名Differentiation-stimulating Factor, D factor, Melanoma-derived LPL Inhibitor , MLPLI

UniprotP09056

Gene ID16878

计算分子量Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

序列SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

配方Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.