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位置: 首页 > 重组蛋白 > Recombinant Murine Acidic Fibroblast Growth Factor

Recombinant Murine Acidic Fibroblast Growth Factor#AP60184

Recombinant Murine Acidic Fibroblast Growth Factor
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Murine Acidic Fibroblast Growth Factor

来源种属Escherichia coli.

纯化> 96 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x106 IU/mg in the presence of 10 μg/ml of heparin.

内毒素Less than 1 EU/ug of rMuaFGF as determined by LAL method.

别名FGF-1, HBGF-1

UniprotP61148

Gene ID14164

计算分子量Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 140 amino acid residues.

序列FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD

配方Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Murine aFGF, encoded by the FGF1 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Murine aFGF shares 52 % amino acid sequence identity with bFGF. Murine aFGF shares 96 % amino acid sequence identity with human aFGF, so it exhibits considerable species crossreactivity between murine and human aFGF. In mammalian FGF receptor family has 4 members, FGFR1, FGFR2, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types 鈥渂鈥? 鈥渃鈥? aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. aFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. aFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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