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位置: 首页 > 重组蛋白 > Recombinant Murine Keratinocyte Growth Factor 1/FGF-7

Recombinant Murine Keratinocyte Growth Factor 1/FGF-7#AP60185

Recombinant Murine Keratinocyte Growth Factor 1/FGF-7
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Murine Keratinocyte Growth Factor 1/FGF-7

来源种属Escherichia coli.

纯化> 96 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x105 IU/mg.

内毒素Less than 1 EU/ug of rMuKGF-1/FGF-7 as determined by LAL method.

别名,HBGF-7

UniprotP36363

Gene ID14178

计算分子量Approximately 18.7 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids.

序列CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT

配方Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Murine KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51 % amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, murine KGF-1 shares 96 % amino acid sequence identity with human and rat.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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