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位置: 首页 > 重组蛋白 > Recombinant Murine Fibroblast Growth Factor 8

Recombinant Murine Fibroblast Growth Factor 8#AP60186

Recombinant Murine Fibroblast Growth Factor 8
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Murine Fibroblast Growth Factor 8

来源种属Escherichia coli.

纯化> 97 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 x105 IU/mg in the presence of 10 μg/ml of heparin.

内毒素Less than 1 EU/ug of rMuFGF-8 as determined by LAL method.

别名AIGF, HBGF-8, FGF-8c

UniprotP37237

Gene ID14179

计算分子量Approximately 28.1 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids.

序列QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR

配方Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Murine FGF-8 is a heparin binding growth factor belonging to the FGF family, which plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Murine FGF-8 is first purified from an androgen-dependent mouse mammary carcinoma cell line as an androgen induces secretion. Cloning and analysis of the murine FGF8 gene revealed at least eight potential protein isoforms (FGF-8a-h). Murine FGF-8a and b share 100 % amino acid identity with that in humans, and murine FGF-8e and f share 98 % amino acid identity with humans. None of the FGF-8 isoforms exhibited activity to FGFR1b, 2b, 3b, but FGFR2c, 3c and FGFR4 can be activated by several FGF-8 isoforms. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration, and it is required for normal brain, eye, ear and limb development during embryogenesis.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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