产品名称Recombinant Rat Tumor Necrosis Factor-alpha/TNFSF2
来源种属Escherichia coli.
纯化> 98 % by SDS-PAGE and HPLC analyses.
生物活性Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x107 IU/mg in the presence of actinomycin D.
内毒素Less than 1 EU/ug of rRtTNF-α as determined by LAL method.
别名Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing Factor , DIF, Necrosin, Cytotoxin
UniprotP16599
Gene ID24835
计算分子量Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
序列LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL
配方Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl.
保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied.
- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.
- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.