产品名称Recombinant Rat Ciliary Neurotrophic Factor
来源种属Escherichia coli.
纯化> 95 % by SDS-PAGE and HPLC analyses.
生物活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg.
内毒素Less than 1 EU/ug of rRtCNTF as determined by LAL method.
别名CNTF
UniprotP20294
Gene ID25707
计算分子量Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.
序列AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
配方Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4, 2 % trehalose.
保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied.
- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.
- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.