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位置: 首页 > 重组蛋白 > Recombinant Equine Interleukin-1 Receptor Antagonist Protein

Recombinant Equine Interleukin-1 Receptor Antagonist Protein#AP60274

Recombinant Equine Interleukin-1 Receptor Antagonist Protein
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Equine Interleukin-1 Receptor Antagonist Protein

来源种属Escherichia coli.

纯化> 95 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 3.0 μg/ml, corresponding to a specific activity of > 333 IU/mg in the presence of 50 pg/ml rHuIL-1α.

内毒素Less than 1 EU/ug of rEqIL-1RA as determined by LAL method.

别名IL-1RN, IRAP

UniprotO18999

Gene ID100034236

计算分子量Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

序列HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ

配方Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. It is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-1RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The equus caballus IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1尾 and 19 % homology to IL-1α. It also shares 78 %~80 % a.a. sequence identity with murine, rat, porcine IL-1RA.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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