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位置: 首页 > 重组蛋白 > Recombinant Human Monocyte Chemotactic Protein-1/CCL2

Recombinant Human Monocyte Chemotactic Protein-1/CCL2#AP60296

Recombinant Human Monocyte Chemotactic Protein-1/CCL2
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Monocyte Chemotactic Protein-1/CCL2

来源种属Escherichia coli.

纯化> 96 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

内毒素Less than 1 EU/ug of rHuMCP-1/CCL2 as determined by LAL method.

别名HC11, Monocyte chemoattractant protein 1, MCAF, MCP-1, Monocyte secretory protein JE, Small-inducible cytokine A2

UniprotP13500

Gene ID6347

计算分子量Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

序列QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

配方Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
CCL2, also named monocyte chemotactic protein-1 (MCP-1) and small inducible cytokine A2, is a small cytokine that belongs to the CC chemokine family. It is a monomeric polypeptide, with a molecular weight of approximately 13 kDa and it is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. Research shows that CCR2 and CCR4 are two cell surface receptors that bind CCL2. CCL2 exhibits a chemotactic activity for monocytes and basophils. However, it does not attract neutrophils or eosinophils. CCL2 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis and it may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Mature human CCL2 shares 78-79 % a.a. identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 a.a. and shares only ~56 % a.a. identity within the common region.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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