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位置: 首页 > 重组蛋白 > Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14

Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14#AP60307

Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Hemofiltrate CC Chemokine-1, 66a.a./CCL14

来源种属Escherichia coli.

纯化> 95 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-20 ng/ml.

内毒素Less than 1 EU/ug of rHuHCC-1, 66a.a./CCL14 as determined by LAL method.

别名C-C Motif Chemokine 14, HCC-3, NCC-2, Small-inducible Cytokine A14

UniprotQ16627

Gene ID6358

计算分子量Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 66 amino acids.

序列GPYHPSECCF TYTTYKIPRQ RIMDYYETNS QCSKPGIVFI TKRGHSVCTN PSDKWVQDYI KDMKEN

配方Lyophilized from a 0.2 μm filtered concentrated solution in 2 x PBS, pH 7.4, 5 % trehalose.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Human CCL14 is belonging to the CC chemokine family. It is encoded by the gene CCL14. CCL14 has two isoforms, CCL14a (HCC-1) and CCL14b (HCC-3). The sequence of HCC-3 differs from HCC-1 as follow: 27-27 R鈫?QTGGKPKVVKIQLKLVG. CCL14 was first isolated from the hemofiltrate of human patients with chronic renal failure. The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. CCL14 promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1 and is a ligand for CCR1, CCR3 and CCR5. Recombinant human CCL14 (66 a.a.) contains 66 amino acid residues and activation of the HCC 1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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