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位置: 首页 > 重组蛋白 > Recombinant Human Ubiquitin-conjugating Enzyme E2 B, His

Recombinant Human Ubiquitin-conjugating Enzyme E2 B, His#AP60434

Recombinant Human Ubiquitin-conjugating Enzyme E2 B, His
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Ubiquitin-conjugating Enzyme E2 B, His

来源种属Escherichia coli.

纯化> 95 % by SDS-PAGE and HPLC analyses.

生物活性Data is not available.

内毒素Less than 1 EU/ug of rHuUBE2B, His as determined by LAL method.

别名HR6B, Ubiquitin Carrier Protein B, Ubiquitin-protein Ligase B

计算分子量Approximately 19.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids of human UBE2B and 14 a.a. vector sequence including 6 x His tag at N-terminus.

序列MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS

配方A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Ubiquitin-conjugating enzyme E2 B belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2B gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2B interacts with RAD18, UBR2 and WAC. Its protein sequence is 100 % identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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