免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 重组蛋白 > Recombinant Human Ubiquitin-conjugating Enzyme E2 L3, His

Recombinant Human Ubiquitin-conjugating Enzyme E2 L3, His#AP60436

是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Ubiquitin-conjugating Enzyme E2 L3, His

来源种属Escherichia coli.

纯化> 95 % by SDS-PAGE and HPLC analyses.

生物活性Data is not available.

内毒素Less than 1 EU/ug of rHuUBE2L3, His as determined by LAL method.

别名L-UBC, UbcH7, Ubiquitin Carrier Protein L3, Ubiquitin-protein Ligase L3

计算分子量Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 x His tag at N-terminus.

序列MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD

配方A 0.2 μm filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Ubiquitin-conjugating enzyme E2 L3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2L3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2L3 specifically acts with HECT-type and RBR family E3 ubiquitin-proteinshown to interact with UBOX5, ARIH1, Cbl gene, UBE3A and NEDD4. Down-regulated during the S-phase it is involved in progression through the cell cycle. Additionally, UBE2L3 regulates nuclear hormone receptors transcriptional activity and plays a role in myelopoiesis.

如果您使用该产品AP60436发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com