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位置: 首页 > 重组蛋白 > Recombinant Human Syndecan-4

Recombinant Human Syndecan-4#AP60451

Recombinant Human Syndecan-4
是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Syndecan-4

来源种属Escherichia coli.

纯化> 95 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The specific activity is determined by binding ability in a functional ELISA. Immobilized rHuSYND4 at 500 ng/ml (100 μl/well) can bind rHubFGF with a linear range of 0.1-10 ng/ml.

内毒素Less than 0.1 EU/ugof rHuSYND4 as determined by LAL method.

UniprotP31431

Gene ID6385

计算分子量Approximately 13.9 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. But it migrates with an apparent molecular mass of 22 kDa in SDS-PAGE.

序列ESIRETEVID PQDLLEGRYF SGALPDDEDV VGPGQESDDF ELSGSGDLDD LEDSMIGPEV VHPLVPLDNH IPERAGSGSQ VPTEPKKLEE NEVIPKRISP VEESEDVSNK VSMSSTVQGS NIFERTE

配方Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Syndecan-4 (SYND4) encoded by the SDC-4 gene in humans, has a molecular weight of ~20 kDa. It is one of the four vertebrate syndecans which belong to the syndecan family of Type 1 transmembrane proteins and capable of carrying heparin sulfate (HS) and chondroitin sulfate glycosaminoglycans. Syndecans are the best-characterized plasma membrane proteoglycans with two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. SYND4 is the most similar to SYND2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGFβ2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator. SYND4 has more widespread distribution than other syndecans and it is the only syndecan that has been found consistently in focal adhesions. Human SYND4 ECD shares approximately 79 %, 78 % and 81% a.a. identity with mouse, rat and porcine SYND4 ECD, respectively.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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