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位置: 首页 > 重组蛋白 > Recombinant Human Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag

Recombinant Human Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag#AP60452

是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag

来源种属Escherichia coli.

纯化> 95 % by SDS-PAGE and HPLC analyses.

内毒素Less than 1 EU/ugof rHuRb137, His as determined by LAL method.

别名p105-Rb, pRb, pp110

计算分子量Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

序列MASFPSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGSNP PKPLKKLRFD IEGSDEADGS KHLPGESKFQ QKLAEMTSTR TRMQKQKMND SMDTSNKEEK HHHHHH

配方Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
Rb encoded by the RB1 gene in humans, is expressed by retina and belongs to the etinoblastoma-associated protein family. The hole protein consists of 928 a.a. and the rHuRb fragment occupies sequence of 792-929 a.a.. Rb is a key regulator of entry into cell division that acts as a tumor suppressor. It has many functions, for example, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C, and acts as a transcription repressor of E2F1 target genes and so on. The rHuRb is the region that rich of modified residue like phosphothreonine and N6-acetyllysine.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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