产品名称Recombinant Human Sonic Hedgehog N-Terminus
来源种属Escherichia coli.
纯化> 98 % by SDS-PAGE and HPLC analyses.
生物活性Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 x103 IU/mg.
内毒素Less than 1 EU/ug of rHuSHH as determined by LAL method.
别名HHG-1
UniprotQ15465
Gene ID6469
计算分子量Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
序列IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
配方Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied.
- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.
- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
3-5days
