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位置: 首页 > 重组蛋白 > Recombinant Human Indian Hedgehog Cys28IleIle

Recombinant Human Indian Hedgehog Cys28IleIle#AP60467

是否有货: 3-5days
产品总价:
产品详情

产品名称Recombinant Human Indian Hedgehog Cys28IleIle

来源种属Escherichia coli.

纯化> 96 % by SDS-PAGE and HPLC analyses.

生物活性Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.

内毒素Less than 1 EU/ug of rHuIHH C28II as determined by LAL method.

别名HHG-2

UniprotQ14623

Gene ID3549

计算分子量Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

序列IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG

配方Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS with 0.02% Tween-20, pH 7.0.

保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied. - 1 month, 2 to 8 ˚C under sterile conditions after reconstitution. - 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.

背景
IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in adult kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH聽is聽also聽involved聽in聽yolk聽sac聽vasculogenesis,聽playing聽an聽important聽role聽in聽differentiation聽of聽epiblast聽cells聽into聽endothelial聽and聽red聽blood聽cells. Human IHH shares 100 % amino acid sequence identity with murine.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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