产品名称Recombinant Human Osteopontin/OPN
来源种属Mouse myeloma cell line, NS0
纯化> 95 % by SDS-PAGE analyses.
生物活性Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37˚C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.
内毒素Less than 0.1 EU/ug of rHuOsteopontin/OPN as determined by LAL method.
计算分子量Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag.
序列Ile17-Asn300, with a C-terminal 6-His tag; Accession # NP_000573.1
配方Lyophilized from 0.2 μm filtered concentrated solution in PBS, pH 7.2.
保存Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months from date of receipt, when stored at ≤-20 ˚C as supplied.
- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.
- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
3-5days
