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位置: 首页 > 磷酸化抗体 > SAPK/JNK(Phospho-Thr183) Antibody

SAPK/JNK(Phospho-Thr183) Antibody#11249

SAPK/JNK(Phospho-Thr183) Antibody
SAPK/JNK(Phospho-Thr183) Antibody
SAPK/JNK(Phospho-Thr183) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称SAPK/JNK(Phospho-Thr183) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性 The antibody detects endogenous level of SAPK/JNK only when phosphorylated at threonine 183.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from Human SAPK/JNK.

基因/蛋白名称SAPK/JNK

修饰Phospho

别名JNK2

数据库入口号Swiss-Prot: P45984/P53779
NCBI Protein: NP_001128516.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 46 54 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells using SAPK/JNK(Phospho-Thr183) Antibody #11249(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK(Phospho-Thr183) Antibody #11249(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from 293 cells, treated with UV or calf intestinal phosphatase (CIP), using SAPK/JNK (Phospho-Thr183) Antibody #11249.
背景

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782

如果您使用该产品11249发表了文章,请通知我们,让我们可以引用您的文献。

et al,Treponema denticola Induces Neuronal Apoptosis by Promoting Amyloid-β Accumulation in Mice. In Pathogens on 2022 Oct 5 by Linrui Wu, Xinyi Su, et al..PMID: 36297207, , (2022),
PMID: 36297207
et al,Berberine ameliorates erectile dysfunction in rats with streptozotocin-induced diabetes mellitus through the attenuation of apoptosis by inhibiting the SPHK1/S1P/S1PR2 and MAPK pathways In Andrology on 2022 Feb by Kang Liu, Taotao Sun, et al..PMID:34674380, , (2022),
PMID: 34674380
Peng Jiao, Yun-Sheng Zhou, Juan-Xia Yang et al,MK-2206 induces cell cycle arrest and apoptosis in HepG2 cells and sensitizes TRAIL-mediated cell death, Mol Cell Biochem, 382(1-2):217-24 (2013),
PMID: 23797319
et al,Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts. In Protein Expr Purif on 2011 Feb by Chati L Zony, Brian J Bahnson, et al..PMID:20709173, , (2011),
PMID: 20709173
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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