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位置: 首页 > 磷酸化抗体 > p70 S6 Kinase(Phospho-Thr421) Antibody

p70 S6 Kinase(Phospho-Thr421) Antibody#11254

p70 S6 Kinase(Phospho-Thr421) Antibody
p70 S6 Kinase(Phospho-Thr421) Antibody
p70 S6 Kinase(Phospho-Thr421) Antibody
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产品总价:
产品详情

产品名称p70 S6 Kinase(Phospho-Thr421) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at threonine 421.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 421 (P-R-T(p)-P-V) derived from Human p70 S6 Kinase.

基因/蛋白名称p70 S6 Kinase

修饰Phospho

别名KS6B1; P70-S6K; RPS6KB1; S6K;

数据库入口号Swiss-Prot: P23443
NCBI Protein: NP_003152.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 70 85 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Jurkat cells untreated(lane 1) or treated with GM-CSF(lane 2) using p70 S6 Kinase(Phospho-Thr421) Antibody #11254.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Phospho-Thr421) Antibody #11254(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using p70 S6 Kinase(Phospho-Thr421) Antibody #11254.
背景
Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Xiao-Feng, et al. (2003) Le1 30 Volume 22: 484-497
An WL, et al. (2003) Am J Pathol. 163(2): 591-607.
Le XF, et al. (2003) Oncogene.22(4): 484-97

如果您使用该产品11254发表了文章,请通知我们,让我们可以引用您的文献。

X Tang, X Zhou, K Zhou et al,Dauricine inhibits insulin-like growth factor-I-induced hypoxia inducible factor 1alpha protein accumulation and vascular endothelial growth factor expression in human breast cancer cells., Acta Pharmacologica Sinica., 30 (5): 605篓C616 (2009),
PMID: 19349962
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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