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位置: 首页 > 磷酸化抗体 > Connexin 43 (phospho-Ser368) Antibody

Connexin 43 (phospho-Ser368) Antibody#11258

Connexin 43 (phospho-Ser368) Antibody
Connexin 43 (phospho-Ser368) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Connexin 43 (phospho-Ser368) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Connexin43 only when phosphorylated at serine 368.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 368 (R-A-S(p)-S-R) derived from Human Connexin 43.

基因/蛋白名称Connexin 43

修饰Phospho

别名CX43; CXA1; CXN-43; GJA1;

数据库入口号Swiss-Prot: P17302
NCBI Protein: NP_000156.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 (Phospho-Ser368) Antibody #11258 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 293 cells untreated or treated with PMA using Connexin 43 (phospho-Ser368) Antibody #11258.
背景

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211
Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353,
Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654
W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444
 

如果您使用该产品11258发表了文章,请通知我们,让我们可以引用您的文献。

et al,A novel role of SIRT2 in regulating gap junction communications via connexin-43 in bovine cumulus-oocyte complexes.InJ Cell Physiol on 2020 Feb 10. by Xu D, He H et al..PMID:32039484 , , (2020),
PMID: 32039484
et al,Electric stimulus opens intercellular spaces in skin.In J Biol Chem.On 2014 Jan 24 by Hama S, Kimura Y et al..PMID:24318878, , (2014),
PMID: 24318878
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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