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位置: 首页 > 磷酸化抗体 > HSF1(phospho-Ser303) Antibody

HSF1(phospho-Ser303) Antibody#11263

HSF1(phospho-Ser303) Antibody
HSF1(phospho-Ser303) Antibody
HSF1(phospho-Ser303) Antibody
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产品总价:
产品详情

产品名称HSF1(phospho-Ser303) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of HSF1 only when phosphorylated at serine 303.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 303 (P-P-S(p)-P-P) derived from Human HSF1.

基因/蛋白名称HSF1

修饰Phospho

别名HSTF 1

数据库入口号Swiss-Prot: Q00613
NCBI Protein: NP_005517.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from MCF7 cells using HSF1(phospho-Ser303) Antibody #11263(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1(Phospho-Ser303) Antibody #11263(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (phospho-Ser303) Antibody #11263.
背景

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Parvaneh Rafiee, et,al. (2006) Am J Physiol Cell Physiol ; 291: C931 - C945
Fumika Shinozaki, et,al. (2006) J. Biol. Chem ; 281: 16361 - 16369.
Eiichi Takaki, et,al. (2006) J. Biol. Chem ; 281: 4931 - 4937.
Jan-Jong Hung, et,al. (1998) J. Biol. Chem ; 273: 31924.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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