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位置: 首页 > 磷酸化抗体 > EGFR(phospho-Tyr1110) Antibody

EGFR(phospho-Tyr1110) Antibody#11264

EGFR(phospho-Tyr1110) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称EGFR(phospho-Tyr1110) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1110.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 1110 (P-V-Y(p)-H-N) derived from Human EGFR.

基因/蛋白名称EGFR

修饰Phospho

别名ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR

数据库入口号Swiss-Prot: P00533
NCBI Protein: NP_005219.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(phospho-Tyr1110) Antibody #11264.
背景
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Kaisa Erjala, et,al. (2006) Clin. Cancer Res Jul 2006; 12: 4103 - 4111.
Judit Anido, et,al. (2003) Clin. Cancer Res ; 9: 1274.
Julian Andreev, et,al. (2001) J. Biol. Chem ; 276: 20130 - 20135.
Suil Kim, et,al. (2002) Am J Physiol Lung Cell Mol Physiol, ; 283: 67.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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