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位置: 首页 > 磷酸化抗体 > Merlin(Phospho-Ser518) Antibody

Merlin(Phospho-Ser518) Antibody#11266

Merlin(Phospho-Ser518) Antibody
Merlin(Phospho-Ser518) Antibody
Merlin(Phospho-Ser518) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Merlin(Phospho-Ser518) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Merlin only when phosphorylated at serine 518.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin.

基因/蛋白名称Merlin

修饰Phospho

别名MERL; NF2; Neurofibromin 2; SCH; Schwannomerlin

数据库入口号Swiss-Prot: P35240
NCBI Protein: NP_000259.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 69kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) using Merlin(Phospho-Ser518) Antibody #11266.
Immunofluorescence staining of methanol-fixed Hela cells using Merlin(Phospho-Ser518) Antibody #11266.
Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Merlin (Phospho-Ser518) Antibody #11266.
背景

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.

Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol ; 25: 2384 - 2394.
Hi-Su Yang, et al. (2006) Cancer Res ; 66: 2708 - 2715.
R Bohni, et al. (1994) J. Biol. Chem ; 269: 14541 - 14545.
Adam J. Ratner, et al. (2001) J. Biol. Chem ; 276: 19267 - 19275.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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