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位置: 首页 > 磷酸化抗体 > Cortactin(Phospho-Tyr466) Antibody

Cortactin(Phospho-Tyr466) Antibody#11272

Cortactin(Phospho-Tyr466) Antibody
Cortactin(Phospho-Tyr466) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Cortactin(Phospho-Tyr466) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN.

基因/蛋白名称Cortactin

修饰Phospho

别名Amplaxin; CTTN; EMS1; SRC8;

数据库入口号Swiss-Prot: Q14247
NCBI Protein: NP_005222.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 85kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using Cortactin(Phospho-Tyr466) Antibody #11272.
Western blot analysis of extracts from JK cells, treated with H2O2 or calf intestinal phosphatase (CIP), using Cortactin (Phospho-Tyr466) Antibody #11272.
背景

Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093
Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229
Li Y, et al. (2000) J Biol Chem ; 275(47): 37187-37193
Kinley AW, et al. (2003) Curr Biol ; 13(5): 384-393

如果您使用该产品11272发表了文章,请通知我们,让我们可以引用您的文献。

et al,Laminin-111 peptide C16 regulates invadopodia activity of malignant cells through 尾1 integrin, Src and ERK 1/2. In Oncotarget on 2016 Jul 26 by Adriane S Siqueira, Monique P Pinto,et al..PMID:27323814, , (2016),
PMID: 27323814
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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