免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > p56Dok-2(Phospho-Tyr299) Antibody

p56Dok-2(Phospho-Tyr299) Antibody#11278

p56Dok-2(Phospho-Tyr299) Antibody
p56Dok-2(Phospho-Tyr299) Antibody
p56Dok-2(Phospho-Tyr299) Antibody
p56Dok-2(Phospho-Tyr299) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p56Dok-2(Phospho-Tyr299) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2.

基因/蛋白名称p56Dok-2

修饰Phospho

别名DOK2

数据库入口号Swiss-Prot: O60496
NCBI Protein: NP_003965.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 56kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Jurkat cells untreated or treated with insulin using p56Dok-2(Phospho-Tyr299) Antibody #11278.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2(Phospho-Tyr299) Antibody #11278(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Phospho-Tyr299) Antibody #11278.
Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody #11278.
背景

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

Feng Cong, et,al. (1999) Mol. Cell. Biol ; 19: 8314 - 8325.
Serge Lemay, et,al. (2000) Mol. Cell. Biol ; 20: 2743 - 2754.
Ute Schaeper, et,al.(2000) J. Cell Biol ; 149: 1419.
Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.

如果您使用该产品11278发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com