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位置: 首页 > 磷酸化抗体 > eIF2a(Phospho-Ser51) Antibody

eIF2a(Phospho-Ser51) Antibody#11279

eIF2a(Phospho-Ser51) Antibody
eIF2a(Phospho-Ser51) Antibody
eIF2a(Phospho-Ser51) Antibody
eIF2a(Phospho-Ser51) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称eIF2a(Phospho-Ser51) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IF,WB,IHC,ELISA   

种属反应性Human,Mouse,Rat,Dog,Pig,Fish

特异性The antibody detects endogenous level of eIF2a only when phosphorylated at serine 51.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.

基因/蛋白名称eIF2a

修饰Phospho

别名Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A;

数据库入口号Swiss-Prot: P05198
NCBI Protein: NP_004085.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a(Phospho-Ser51) Antibody #11279.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51) Antibody #11279 (left) or the same antibody preincubated with blocking peptide #51279 (right).
Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) Antibody #11279.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2α (Phospho-Ser51) Antibody #11279.
背景

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460.
Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992.
Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.

如果您使用该产品11279发表了文章,请通知我们,让我们可以引用您的文献。

et al,Mechanisms of impaired pancreatic β?cell function in high?fat diet?induced obese mice: The role of endoplasmic reticulum stress.In Mol Med Rep on 2020 May; by Yi X, Cai X, et al..PMID:32323766 , , (2020),
PMID: 32323766
et al,Suppressing endoplasmic reticulum stress-related autophagy attenuates retinal light injury. In Aging (Albany NY) on 2020 Aug 28 by Jing-Yao Song, Bin Fan,et al..PMID: 32858529, , (2020),
PMID: 32858529
et al,螖2-Troglitazone promotes cytostatic rather than pro-apoptotic effects in breast cancer cells cultured in high serum conditions. In Cell Cycle on 2016 Dec 16 by Audrey Berthe, St茅phane Flament,et al..PMID: 27753533, , (2016),
PMID: 27753533
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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