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位置: 首页 > 磷酸化抗体 > Gab1(Phospho-Tyr627) Antibody

Gab1(Phospho-Tyr627) Antibody#11291

Gab1(Phospho-Tyr627) Antibody
Gab1(Phospho-Tyr627) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Gab1(Phospho-Tyr627) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms

特异性The antibody detects endogenous level of Gab1 only when phosphorylated at tyrosine 627.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 627 (V-E-Y(p)-L-D) derived from Human Gab1.

基因/蛋白名称Gab1

修饰Phospho

别名Growth factor receptor bound protein 2-associated protein 1

数据库入口号Swiss-Prot: Q13480
NCBI Protein: NP_002030.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Gab1(Phospho-Tyr627) Antibody #11291.
Immunofluorescence staining of methanol-fixed Hela cells using Gab1(Phospho-Tyr627) Antibody #11291.
背景
The protein encoded by Gab1 is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904.
Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307.
Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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