免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > MEK1(Phospho-Thr291) Antibody

MEK1(Phospho-Thr291) Antibody#11294

MEK1(Phospho-Thr291) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称MEK1(Phospho-Thr291) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of MEK1 only when phosphorylated at threonine 291.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 291 (P-R-T(p)-P-G derived from Human MEK1.

基因/蛋白名称MEK1

修饰Phospho

别名ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1

数据库入口号Swiss-Prot: Q02750
NCBI Protein: NP_002746.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 45kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HepG2 cells untreated or treated with PMA using MEK1(Phospho-Thr291) Antibody #11294.
背景
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Kevin D. Burroughs, et,al. (2003) Mol. Cancer Res ; 1: 312.
Michael J. Piatelli, et,al. (2002) J. Biol. Chem ; 277: 12144 - 12150.
Margaret M. Morgan, et,al. (2001) J. Immunol ; 167: 5708.
Herbert Schramek, et,al. (2003) Am J Physiol Cell Physiol, ; 285: C652 - C661.

如果您使用该产品11294发表了文章,请通知我们,让我们可以引用您的文献。

Akira Ikari, Kosuke Atomi, Keishi Kinjo et al,Magnesium deprivation inhibits a MEK篓CERK cascade and cell proliferation in renal epithelial Madin-Darby canine kidney cells., Life Sciences, 86: 766篓C773 (2010),
PMID: 20338184
Hanqian Xu, Gan Zhao, Xiaoxi Huang et al,CD40-expressing plasmid induces anti-CD40 antibody and enhances immune responses to DNA vaccination., The Journal of Gene Medicine., 12(1)97-106 (2010),
PMID: 19950201
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com