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位置: 首页 > 磷酸化抗体 > PKCth(Phospho-Ser676) Antibody

PKCth(Phospho-Ser676) Antibody#11297

PKCth(Phospho-Ser676) Antibody
PKCth(Phospho-Ser676) Antibody
PKCth(Phospho-Ser676) Antibody
PKCth(Phospho-Ser676) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PKCth(Phospho-Ser676) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of PKCth only when phosphorylated at serine 676.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKCth.

基因/蛋白名称PKCth

修饰Phospho

别名KPCT; PKC-theta; PKCQ; PRKCQ; PRKCT

数据库入口号Swiss-Prot: Q04759
NCBI Protein: NP_006248.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCth(Phospho-Ser676) Antibody #11297.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser676) Antibody #11297(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Phospho-Ser676) Antibody #11297.
Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKCθ (Phospho-Ser676) Antibody #11297.
背景

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.

Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253.
Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123.
Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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