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位置: 首页 > 磷酸化抗体 > IkB-b(Phospho-Ser23) Antibody

IkB-b(Phospho-Ser23) Antibody#11304

IkB-b(Phospho-Ser23) Antibody
IkB-b(Phospho-Ser23) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称IkB-b(Phospho-Ser23) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of IkB-b only when phosphorylated at serine 23.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 23 (L-G-S(p)-L-G) derived from Human IkB-b.

基因/蛋白名称IkB-b

修饰Phospho

别名I-kappa-B-beta; IKB-B; IKBB; NF-kappa-BIB; NF-kappaB inhibitor beta

数据库入口号Swiss-Prot: Q15653
NCBI Protein: NP_001001716.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 48kd
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-b(Phospho-Ser23) Antibody #11304(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from HT29 cells, treated with CA or calf intestinal phosphatase (CIP), using IκB-β (Phospho-Ser23) Antibody #11304.
背景

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174.
DiDonato J, et al. (1996) Mol Cell Biol 16(4): 1295-304

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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