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位置: 首页 > 磷酸化抗体 > Keratin 18(Phospho-Ser33) Antibody

Keratin 18(Phospho-Ser33) Antibody#11306

Keratin 18(Phospho-Ser33) Antibody
Keratin 18(Phospho-Ser33) Antibody
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产品总价:
产品详情

产品名称Keratin 18(Phospho-Ser33) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 33 (A-A-S(p)-V-Y) derived from Human Keratin 18 (CK18).

基因/蛋白名称Keratin 18

修饰Phospho

别名CK 18; CK18; CYK18; Cytokeratin endo B; K18

数据库入口号Swiss-Prot: P05783
NCBI Protein: NP_000215.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Keratin 18(Phospho-Ser33) Antibody #11306.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18(Phospho-Ser33) Antibody #11306(left) or the same antibody preincubated with blocking peptide(right).
背景
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
Moll, R. et al. (1982) Cell 31, 11-24.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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