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位置: 首页 > 磷酸化抗体 > SHP-1(Phospho-Tyr536) Antibody

SHP-1(Phospho-Tyr536) Antibody#11318

SHP-1(Phospho-Tyr536) Antibody
SHP-1(Phospho-Tyr536) Antibody
SHP-1(Phospho-Tyr536) Antibody
SHP-1(Phospho-Tyr536) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称SHP-1(Phospho-Tyr536) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of SHP-1 only when phosphorylated at tyrosine 536.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 536 (S-E-Y(p)-G-N) derived from Human SHP-1.

基因/蛋白名称SHP-1

修饰Phospho

别名70Z-SHP; HCP; HCPH; Hematopoietic cell protein-tyrosine phosphatase; PTN6

数据库入口号Swiss-Prot: P29350
NCBI Protein: NP_002822.2

浓度1.0mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

WB 1:500 - 1:2000.
IHC 1:100 - 1:300 .
IF 1:50-200

Western Blot analysis of RAW264.7+EGF cells
Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 5'. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma. The picture on the right is blocked with the phospho peptide.
背景

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Migone TS, et al. (1998) Proc Natl Acad Sci USA; 95(7): 3845-3850.
Timms JF, et al.(1998) Mol Cell Biol; 18(7): 3838-3850.
Kanagasundaram V, et al. (1999) Mol Cell Biol; 19(6): 4079-4092.
Hauck CR, et al. (1999) Infect Immun; 67(10): 5490-5494.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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