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位置: 首页 > 磷酸化抗体 > Smad2(Phospho-Ser467) Antibody

Smad2(Phospho-Ser467) Antibody#11322

Smad2(Phospho-Ser467) Antibody
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产品总价:
产品详情

产品名称Smad2(Phospho-Ser467) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Smad2 only when phosphorylated at serine 467.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 467 (C-S-S-M-S(p)) derived from Human Smad2.

基因/蛋白名称Smad2

修饰Phospho

别名JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2

数据库入口号Swiss-Prot: Q15796
NCBI Protein: NP _001003652.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 60kd
Western blotting: 1:500~1:1000

IHC 1:50-1:200

Western blot analysis of extracts from Hela cells untreated or treated with PMA using Smad2(Phospho-Ser467) Antibody #11322.
背景

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

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Xing-Yi Zhang, Bao-Rong Shen, Yu-Cheng Zhang et al,Induction of Thoracic Aortic Remodeling by Endothelial-Specific Deletion of MicroRNA-21 in Mice., PLoS ONE., 8(3): e59002. doi:10.1371/journal.pone.0059002 (2013),
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PMID: 23527070
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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