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位置: 首页 > 磷酸化抗体 > a-catenin(Phospho-Ser641) Antibody

a-catenin(Phospho-Ser641) Antibody#11330

a-catenin(Phospho-Ser641) Antibody
a-catenin(Phospho-Ser641) Antibody
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产品总价:
产品详情

产品名称a-catenin(Phospho-Ser641) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of a-catenin only when phosphorylated at serine 641.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 641 (D-D-S(p)-D-F) derived from Human a-catenin.

基因/蛋白名称a-catenin

修饰Phospho

别名Cadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen

数据库入口号Swiss-Prot: P35221
NCBI Protein: NP_001894.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 100kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Hela cells untreated or treated with starvation using a-catenin(Phospho-Ser641) antibody #11330.
Western blot analysis of extracts from HT29 cells, treated with serum or calf intestinal phosphatase (CIP), using α-catenin (Phospho-Ser641) Antibody #11330.
背景

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation.

Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765
Drees, F. et al. (2005) Cell 123, 903-915.
Yamada, S. et al. (2005) Cell 123, 889-901.
Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.

如果您使用该产品11330发表了文章,请通知我们,让我们可以引用您的文献。

et al,Ginsenoside Rh2 activates α-catenin phosphorylation to inhibit lung cancer cell proliferation and invasion.In Exp Ther Med on 2020 Apr; by Zhang G, He L, et al..PMID: 32256776, , (2020),
PMID: 32256776
et al,2?Aminothiazole Derivatives as Selective Allosteric Modulators of the Protein Kinase CK2. 2. Structure-Based Optimization and Investigation of Effects Specific to the Allosteric Mode of Action.In J Med Chem.On 2019 Feb 28 by Bestgen B, Kufareva I et al..PMID:30689946, , (2019),
PMID: 30689946
et al,Force-dependent allostery of the α-catenin actin-binding domain controls adherens junction dynamics and functions.In Nat Commun. On 2018 Nov 30 by Ishiyama N, Sarpal R et al..PMID: 30504777, , (2018),
PMID: 30504777
et al,Helicobacter pylori Induces Cell Migration and Invasion Through Casein Kinase 2 in Gastric Epithelial Cells.In Helicobacter on 2014 Dec by Yeo Song Lee, Do Yeon Lee et al..PMID: 25052887, , (2014),
PMID: 25052887
et al,EGFR-induced and PKC蔚 monoubiquitylation-dependent NF-魏B activation upregulates PKM2 expression and promotes tumorigenesis. In Mol Cell on 2012 Dec 14 by Weiwei Yang, Yan Xia, et al..PMID: 23123196, , (2012),
PMID: 23123196
Ji H, Wang J, Nika H et al,EGF-induced ERK activation promotes CK2-mediated disassociation of alpha-Catenin from beta-Catenin and transactivation of beta-Catenin., Mol Cell, 36(4):547-559 (2009),
PMID: 19941816
et al,EGF-induced ERK activation promotes CK2-mediated disassociation of alpha-Catenin from beta-Catenin and transactivation of beta-Catenin. In Mol Cell on 2009 Nov 25 by Ji H, Wang J,et al..PMID:19941816, , (2009),
PMID: 19941816
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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